FLxFlow is a Portuguese network for Flow Cytometry aiming to bring together core facilities in the Lisbon area (Instituto Gulbenkian de Ciência, Instituto de Medicina Molecular João Lobo Antunes, Champalimaud Foundation, NOVA Medical School and iMed.ULisboa – Instituto de Investigação do Medicamento. The network highlights flagships from each location, raising the awareness and facilitating access of scientists for cutting edge applications.