The 2nd FLxFlow Course covered the fundamentals of flow cytometry technology and built up the knowledge of the attendees through a series of lectures, exercises, practical examples and interactive learning. The course was designed to ensure that the attendees were confident to design, carry out, analyse and present their flow cytometry data. It consisted of three modules: basic, advanced and hands-on.
The 1st FLxFlow course introduced the basic principles and applications of flow cytometry to beginners. From fluorophores and panel design, instruments operation, required controls and statistical analysis to data publication and potential applications. The course consisted of theoretical talks and practical sessions with remote teaching where students learned how to set up an experiment, run a multi-color experiment, perform compensation and analyze data. There were also practical hands-on sessions in immunophenotyping, cell cycle, cell death & apoptosis and imaging flow cytometry.