4th FLxFlow Course

This course will cover basic and advanced cytometry topics and comprise lectures and practical modules taught by national and international experts in the field. Cytometry applications will be offered at all levels, targeted at both beginners and advanced users, and will be taught on different instruments in hands-on sessions. This will be an international course that last editions received over 300 participants from Portuguese and from foreign academic and non-academic institutions.


FLxFlow Team

Invited Speakers


Topics to be discussed: 

Basic Module (8-9th May - whole day): 
- Principles of Flow Cytometry
- Fluorochromes 
- Brightness, Detection and Resolution 
- Spillover, compensation and spectral unmixing 
- Panel Design 
- Considerations for planning a flow cytometry experiment 
- Sample Preparation 
- Applications (DNA analysis, proliferation, cell death and apoptosis) 
- Data analysis (I) 

Advanced Module (10th May - whole day; 11-12th May mornings): 
- Advanced Experimental Planning and Troubleshooting 
- Full Spectrum Flow Cytometry 
- Imaging Flow Cytometry 
- Cell sorting 
- Small Particle Analysis 
- Clinical Flow Cytometry 
- Data analysis (II) and Publication 

Hands-On Module (11-12th May - afternoons)


Click here to register


Champalimaud Centre for the Unknown
Avenida Brasília,
1400-038 Lisbon, Portugal

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