Our Facility
The Flow Cytometry Facility at the Instituto Gulbenkian de Ciência (IGC) offers flow cytometry services and expertise to IGC researchers, as well as to outside groups and companies.
Laboratory staff is well trained in instrument operation, providing support to cell sorting, cell acquisition and data analysis. Training is available for all analytical instruments and SOP are implemented to comply with the highest quality standards required to ensure reproducibility in science.
Equipment
Flow Cytometry Analyzers
- BD LSR Fortessa SORP – 3 lasers 488nm, 442nm and 561nm with a FSC PMT and High Throughput Sampler (HTS) option (BSL-1 lab)
- BD LSR Fortessa X20 – 4 lasers 405nm, 488nm, 561 and 640nm (BSL-1 lab)
- CYTEK Aurora – 4 lasers 405nm, 488nm, 561 and 640nm (BSL-1 lab)
Flow Cytometry Sorters
- BD FACSAria II – 3 lasers 405nm, 488nm and 633nm (BSL-2 cabinet)
- CYTEK Aurora CS – 5 lasers 355nm, 405nm, 488nm, 561nm and 640nm (BSL-2 cabinet)
Services
- Cell sorting
- Sample preparation for flow cytometry
- Acquisition of samples in the flow cytometry analyzers
- Flow cytometry data analysis
- Training in flow cytometry
- Experimental consulting for flow cytometry-related projects
- Multicolor antibody panel design and validation for flow cytometry
- Fluorescent conjugation of antibodies or proteins
Staff
Marta Monteiro, PhD – Facility Head
Beatriz Teixeira, MSc – Flow Cytometry Research Support Specialist
Telma Costa, MSc – Flow Cytometry Research Support Specialist
Location
Instituto Gulbenkian de Ciência
Rua da Quinta Grande, 6
2780-156 Oeiras, Portugal
