IGC

Our Facility

The Flow Cytometry & Antibodies Facility at the Instituto Gulbenkian de Ciência (IGC) offers flow cytometry and antibodies-related services and expertise to IGC researchers, as well as to outside groups and companies.

Laboratory staff is well trained in instrument operation, providing support to cell sorting, cell acquisition and data analysis. Full service is provided for antibodies-related requests and include production of monoclonal antibodies, antibody purification and fluorescent labeling of antibodies and proteins. Training is available for all analytical instruments and SOP are implemented to comply with the highest quality standards required to ensure reproducibility in science.

Equipment

Flow Cytometry Analyzers

  • BD FACSCalibur – 2 lasers 488nm and 635 nm (BLS-3 lab)
  • BD LSR Fortessa SORP – 3 lasers 488nm, 442nm and 561nm with a FSC PMT and High Throughput Sampler (HTS) option (BSL-1 lab)
  • BD LSR Fortessa X20 – 4 lasers 405nm, 488nm, 561 and 640nm (BSL-1 lab)
  • CYTEK Aurora – 4 lasers 405nm, 488nm, 561 and 640nm (BSL-1 lab)

Flow Cytometry Sorters

  • BD FACSAria II – 3 lasers 405nm, 488nm and 633nm (BSL-2 cabinet)

Other instruments

  • Agilent Seahorse XFe96 Analyzer – for real-time cell metabolic analysis
  • AKTA Pure – chromatography-based system for fast purification of proteins, peptides, and nucleic acids
  • MAGPIX – multiplex plate reader to quantify simultaneously multiple soluble targets in a single biological sample.

Services

  • Cell sorting
  • Sample preparation for flow cytometry
  • Acquisition of samples in the flow cytometry analyzers
  • Flow cytometry data analysis
  • Training in flow cytometry
  • Experimental consulting for flow cytometry-related projects
  • Multicolor antibody panel design and validation for flow cytometry
  • Mycoplasma testing of cell cultures
  • Production of monoclonal antibodies
  • Fluorescent conjugation of antibodies or proteins
  • Protein purification
  • Real-Time Cell Metabolic Analysis

Staff

 Marta Monteiro, PhD – Facility Head

 Beatriz Teixeira, MSc – Flow Cytometry Research Support Specialist

For more information, please visit our webpage and follow us on Twitter (@IGCFlowFacility).

Location

Instituto Gulbenkian de Ciência
Rua da Quinta Grande, 6
2780-156 Oeiras, Portugal