This course will cover basic and advanced cytometry topics and comprise lectures and practical modules taught by national and international experts in the field. Cytometry applications will be offered at all levels, targeted at both beginners and advanced users, and will be taught on different instruments in hands-on sessions. This will be an international course that last editions received over 300 participants from Portuguese and from foreign academic and non-academic institutions.

Speakers

FLxFlow Team

• André Mozes (Champalimaud Foundation)
• Cláudia Andrade (NOVA Medical School)
• Mariana Fernandes (Instituto de Medicina Molecular João Lobo Antunes)
• Marta Monteiro (INstituto Gulbenkian de Ciência)

Invited Speakers

• Andrew Filby (Newcastle University)
• Catarina Martins (NOVA Medical School)
• Joanne Lannigan (Flow Cytometry Support Services, LLC)
• José Rino (Instituto de Medicina Molecular João Lobo Antunes)
• Lola Martinez (Spanish National Cancer Research Center)

Program

Topics to be discussed: 

Basic Module (8-9th May - whole day): 
- Principles of Flow Cytometry
- Fluorochromes 
- Brightness, Detection and Resolution 
- Spillover, compensation and spectral unmixing 
- Panel Design 
- Considerations for planning a flow cytometry experiment 
- Sample Preparation 
- Applications (DNA analysis, proliferation, cell death and apoptosis) 
- Data analysis (I) 

Advanced Module (10th May - whole day; 11-12th May mornings): 
- Advanced Experimental Planning and Troubleshooting 
- Full Spectrum Flow Cytometry 
- Imaging Flow Cytometry 
- Cell sorting 
- Small Particle Analysis 
- Clinical Flow Cytometry 
- Data analysis (II) and Publication 

Hands-On Module (11-12th May - afternoons)

Registration

Click here to register

Venue

Champalimaud Centre for the Unknown
Avenida Brasília,
1400-038 Lisbon, Portugal

Sponsored By

If you are interested in sponsoring FLxFlow Course, please fill the form:

https://bit.ly/FLxFlow2023_Sponsorship