Andreia Santos

Andreia Santos has a BSc in Biology and a MSc in Genetic and Molecular Biology from Faculdade de Ciências, Universidade de Lisboa.

Andreia joined the iMM Flow Cytometry Unit in September 2017 after working at Ana Espada de Sousa’s lab on human T-cell development, namely the role of IL-4 in the lineage plasticity of human CD4 single positive thymocytes.

Andreia is currently a Flow Cytometry Specialist at the Gulbenkian Institute for Molecular Medicine. She is responsible for cell sorting operation and provides training and support to facility users in the independent use of cell sorters and cell analyzers, including troubleshooting and technical guidance. She has a particular interest in developing and optimizing new flow cytometry panels for a wide range of research applications.

Rute Gonçalves

Rute Gonçalves has a BSc in Biochemistry from University of Coimbra and a MSc in Molecular Genetics and Biomedicine from Universidade Nova de Lisboa.

Rute joined the iMM Flow Cytometry Unit in December 2018, after working at the Immunobiology and Pathogenesis Lab at CEDOC. She was also responsible for CEDOC’s Flow Cytometry Facility from April 2018 to October 2018, providing cell sorting services, supporting researchers and performing all the daily equipment maintenance and quality control.

Rute is currently Manager (Lisbon Site) and Flow Cytometry Specialist at the Gulbenkian Institute for Molecular Medicine, with extensive experience in flow cytometry and high-speed cell sorting, including the operation and troubleshooting of spectral analyzers and image-enabled cell sorters. She supports researchers through all stages of their projects, from experimental design to sample acquisition, data interpretation, and flow cytometry data analysis.

Inês Monteiro

Inês Monteiro began her academic journey at the Faculty of Sciences of the University of Porto, where she completed a bachelor’s degree in Biology. During her studies, she gained early research experience in the Differentiation and Cancer group at i3S, focusing on gastric cancer and the m6A mRNA methylation. She later earned a master’s degree in Molecular and Cellular Biology from the University of Aveiro. For her dissertation, she studied the role of HMGA1 protein in colon cancer stem cells, developing skills in several molecular and cellular biology techniques.

Currently, she works as a Flow Cytometry Technician at the Champalimaud Foundation, where she supports the flow cytometry platform through cell sorting, data analysis, training, and instrument setup.

FLxFlow Webinar 2026

On the 2026 FLxFlow Webinar, we will have Rui Gardner, PhD, Director of the Flow Cytometry Core Facility at Memorial Sloan Kettering Cancer Center, who will explore how imaging-enabled flow cytometry is expanding the capabilities of modern single-cell analysis.

This webinar is particularly relevant for the FLxFlow community, as these cutting-edge imaging flow cytometry technologies are now available within the network through the BD FACSDiscover™ A8 at NOVA Medical School and the BD FACSDiscover™ S8 at GIMM. The session will provide an excellent opportunity to discover the potential of these platforms and learn how they can support innovative research applications.

Date: Thursday, 9 July 2026 | 2:30 PM (Lisbon Time) | Online session

Registration is required:  https://forms.gle/ZLCu7QLiUrki42Lk7

Title: From Images to Insights: Expanding Flow Cytometry Applications with BD CellView™ and FACSDiscover™ Technologies

Speaker:

Rui Gardner, PhD
Director of the Flow Cytometry Core Facility
Memorial Sloan Kettering Cancer Center, New York

Abstract:

The recently developed BD CellView™ Image technology incorporated in the FACSDiscover™ platform has been transformative by merging high-dimensional spectral flow cytometry with imaging collection, enabling researchers around the world to explore further dimensions beyond immunophenotyping, including cell-cell interactions, internalization, co-localization, among others. In this short webinar, we will provide a glance into some of the applications we have been developing within our research institution, including some novel tools for imaging analysis within flow cytometry data.



About the Speaker:

Rui Gardner, PhD is the Director of the Flow Cytometry Core Facility at Memorial Sloan Kettering Cancer Center, New York.

Rui Gardner, began his scientific journey as a mathematical biologist. With a background in Biochemistry, he assumed the role of Head of the Flow Cytometry Core Facility at Instituto Gulbenkian de Ciência in 2007. Overseeing 40+ research groups spanning diverse fields like Immunology, Stem-Cell Biology, Microbiology, and others, Rui brings extensive experience in various Flow Cytometry techniques. His interdisciplinary background in Mathematics, Physics, and Biology allows him to bridge the gap between operational and technical aspects, emphasizing the scientific applications of flow cytometry. Rui is a passionate speaker, having lectured numerous courses on Flow Cytometry and Cell Sorting, and actively contributes to organizing workshops and meetings on Flow Cytometry and Core Management in Europe.

6th FLxFlow Course

This course will cover basic and advanced cytometry topics and comprise lectures and practical modules taught by national and international experts in the field. Cytometry applications will be offered at all levels, targeted at both beginners and advanced users, and will be taught on different instruments in hands-on sessions. This will be an international course that last editions received over 450 participants from Portuguese and from foreign academic and non-academic institutions.

Speakers

FLxFlow Team

Invited Speakers

Preliminary Program

Registration

Coffee-Breaks and Lunches are included for all participants.

On Thursday and Friday lunch is included only for participants selected for the Hands-On Module.

Click here to register – CLOSED

Venue

NMS – Advanced Education Carcavelos Campus
Rua de Luanda 166
2775-233 Parede, Portugal

Sponsored By

If you are interested in sponsoring FLxFlow Course, please fill the form:

https://forms.gle/txvqwrQW2vpdBZo3A

OMIQ Webinar

On April 14th from 2:00 to 4:00 pm we will have Jamie McCarthy, Technical Application Scientist for De Novo Software and OMIQ, giving us a detailed introduction to the powerful OMIQ software.

This online live session is organized by FLxFlow with Dotmatics is free of charge but requires registration. If you wish to attend, please fill the registration form here:

https://forms.gle/pRaUEivc9s5YjU5GA

Registration closes on April 13th.

Cytolution User Meeting

The FLxFlow team is pleased to announce that we are launching two great initiatives to not only promote the use of the advanced analysis software Cytolution, recently acquired by the FLxFlow Flow Cytometry Platforms, but also to support existing users with fresh updates and regular guidance.

1) Cytolution Mailing List

Together with Cytolytics, we have created a mailing list for anyone interested in following Cytolution more closely:

https://fbad3.share-eu1.hsforms.com/2NKqRK2ZzSP2CnSIppSzySw

This mailig list will primarily be used to showcase Cytolution features and share helpful resources on a regular basis. It’s a great way to get to know the software better, and the more familiar you are with it, the more confident you’ll feel using it.

If you want to stay up to date with the latest Cytolution news and tips, make sure to sign up!

2) First Cytolution Users Meeting in Portugal

We are also excited to announce the first Cytolution Users Meeting in Portugal, which will take place at the Champalimaud Foundation on the afternoon of March 24th in the Classroom.

Join the FLxFlow and the Cytolytics teams for an afternoon of deep learning focused on unsupervised data analysis!

Registration is free of charge but mandatory as limited spots are available:

https://forms.gle/AwHojNKfhFUH1cVo7

Dissemination & Promotion

Conferences & Congresses

FLxFlow-HDSpect: Building a Collaborative Infrastructure for Advanced Spectral Flow Cytometry in Portugal

  • XVIII Congress of the Iberian Society of Cytometry (May 14 -16, 2025, Porto, Portugal) 
  • 38th Annual Congress of the International Society for the Advancement of Cytometry (May 31 – June 4, 2025, Denver, USA)
  • LS4FUTURE 2nd General Meeting  (July 1, 2025, Oeiras, Portugal)
  • Nordic Flow Cytometry Meeting (August 20-22, 2025, Copenhagen, Denmark)
  • BFCS2026 – Baltic Flow Cytometry Society Meeting (March 19-20, 2026, Riga, Latvia)

FROM DATA TO INSIGHT: Navigating Complexity as an Emerging Challenge for Flow  Cytometry Platforms 

  • 38th Annual Congress of the International Society for the Advancement of Cytometry (May 31 – June 4, 2025, Denver, USA)

Creating and Optimizing a Panel Design Service by a Flow Cytometry Platform

  • Nordic Flow Cytometry Meeting (August 20-22, 2025, Copenhagen, Denmark)

Levelling the scale: Developing an integrative workflow for isolation of small particles by sorting

  • BFCS2026 – Baltic Flow Cytometry Society Meeting (March 19-20, 2026, Riga, Latvia)

Public Engagement & Outreach Activities

  • NMS Open Day 2025 (May 9, 2025, Lisbon, Portugal)
  • Science Fair – Summer Medical School 2025 (July 10, 2025, Lisbon, Portugal)

2025

December 10th, 9h30

We will have with us Andrea Canto Méndez, Ph.D., a Flow Panel Design Technical Application Scientist at Thermo Fisher Scientific , who will talk about: Unlocking Multicolor Flow Cytometry: Panel Design and Best Practices for Success.

Please register here: https://forms.gle/MpQbC2Bz968MTqVN8

October 15th, 9h30

We will have with us Andrea Gómez, Product Manager from Palex, who will talk about: New Fluorophores in Action – Let’s discover the newest Fluorophores from Biolegend!

Please register here: https://forms.gle/abPx1eCXhaxpBvqw5

May 7th, 9h30

We will have with us Prabhjoat Singh Chana, from ThinkCyte who will talk about ThinkCyte’s VisionSort.

Please register here: https://forms.gle/sJbzxKaG2XnrkiMU6


March 19th, 9h30

We will have with us Alexandre Salvador, from Enzifarma who will talk about BD Horizon Real™ reagents.

Please register here: https://forms.gle/VcTrm4cxXxnLg5kh6

5th FLxFlow Course

This course will cover basic and advanced cytometry topics and comprise lectures and practical modules taught by national and international experts in the field. Cytometry applications will be offered at all levels, targeted at both beginners and advanced users, and will be taught on different instruments in hands-on sessions. This will be an international course that last editions received over 400 participants from Portuguese and from foreign academic and non-academic institutions.

Speakers

FLxFlow Team

Invited Speakers

Program

Registration

FMUL/CAML PhD Students – Free

Applications will be submitted on https://fenix.medicina.ulisboa.pt

Applicants who already have a @campus.ul.pt account (who are attending or have attended a course at the Faculdade de Medicina), must start the application at https://fenix.medicina.ulisboa.pt/application.

To access must log in with the Campus account and then access the Candidate menu (at the top of the page) to start the application.

If you don’t remember the username or password of the account click HERE and follow the instructions for the recovery of the username or password.  If you cannot retrieve your username or password trough the recovery mechanism, you should send an email to the address suporte@medicina.ulisboa.pt with the following data: full name; student number; course number identification; ID number (BI/CC); External email.

Applicants who do not have a @campus.ul.pt account (Fenix) should register HERE. After creating your account you will receive in your email the username after which can start the formalization of the application in https://fenix.medicina.ulisboa.pt.

For further questions please contact: camlphd@medicina.ulisboa.pt

Venue

Gulbenkian Institute for Molecular Medicine – Lisbon Site
Edifício Egas Moniz
Avenida Professor Egas Moniz
1649-028 Lisboa, Portugal

Sponsored By

If you are interested in sponsoring FLxFlow Course, please fill the form:

https://forms.gle/Sw8Jb9f4nMx8LkD6A